PICRUSt: Phylogenetic Investigation of Communities by Reconstruction of Unobserved States

PICRUSt (pronounced “pie crust”) is a bioinformatics software package designed to predict metagenome functional content from marker gene (e.g., 16S rRNA) surveys and full genomes.

PICRUSt is freely available under the GPL.

Click here to go to the PICRUSt GitHub repository.

Please note that PICRUSt2 is now available. PICRUSt2 is a re-written version of PICRUSt and is available here. We are no longer developing PICRUSt1 and we recommend users shift to PICRUSt2.


If you’re new to PICRUSt, you’ll want to work through these documents in order:

More advanced users may be interested in the following (in no particular order):


For PICRUSt announcements and questions, including notification of new releases, you should subscribe to the PICRUSt users list.

Citing PICRUSt

On this page we’ve compiled both the PICRUSt citation and links to several tools that PICRUSt is built on to make citing these various software packages easier.

Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Langille, M. G.I.*; Zaneveld, J.*; Caporaso, J. G.; McDonald, D.; Knights, D.; a Reyes, J.; Clemente, J. C.; Burkepile, D. E.; Vega Thurber, R. L.; Knight, R.; Beiko, R. G.; and Huttenhower, C. Nature Biotechnology, 1-10. 8 2013.

The manuscript describing PICRUSt can be found here

  • Additional citation resources: here

News & Announcements

  • 06-24-19: Official release of PICRUSt 1.1.4
  • 12-06-17: Official release of PICRUSt 1.1.3
  • 08-30-17: Official release of PICRUSt 1.1.2
  • More News & Announcements